Automated particle correspondence and accurate
tilt-axis detection in tilted-image pairs


Single Particle Analysis deals with the problem of reconstructing a three dimensional structure of a macromolecule, e.g. protein-protein or protein-RNA complexes, starting from two dimensional images acquired through transmission electron microscopy.
Tilted electron microscope images are routinely collected for an ab initio structure reconstruction as a part of the Random Conical Tilt (RCT) or Orthogonal Tilt Reconstruction (OTR) methods, as well as for various applications using the "free-hand" procedure. These procedures all require identification of particle pairs in two corresponding images as well as accurate estimation of the tilt-axis used to rotate the electron microscope (EM) grid. We have developed a computational approach, PCT (particle correspondence from tilted pairs), based on tilt-invariant context and projection matching that addresses both problems. The method benefits from treating the two problems as a single optimization task. It automatically finds corresponding particle pairs and accurately computes tilt-axis direction even in the cases when EM grid is not perfectly planar.

[ code ] [ data ]


If you use the resources in this page, please cite our paper:

* "Automated particle correspondence and accurate tilt-axis detection in tilted-image pairs"
Maxim Shatsky*, Pablo Arbelaez*, Bong-Gyoon Han, Dieter Typke, Steven E. Brenner, Jitendra Malik, Robert M. Glaeser.
Journal of Structural Biology, 2014. (* equal contribution)
[ online paper ] [ pdf ]

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