Myoungseok Kim and Grigory Tikhomirov

EECS Department, University of California, Berkeley

Technical Report No. UCB/EECS-2023-237

November 21, 2023

http://www2.eecs.berkeley.edu/Pubs/TechRpts/2023/EECS-2023-237.pdf

Structural DNA nanotechnology has emerged as a versatile approach to building nanometer-precise structures for arranging non-DNA optical nanomaterials. To enable complex optical functions, systematic assembly rules for DNA assemblies into 2D and 3D nanostructures have been developed. However, the low purity and yield of the target superstructure is a major limitation for further development and practical applications. Here, inspired by oligonucleotide synthesis on the solid support, we propose a sequential assembly of DNA structures using the magnetic beads. The iterative addition of DNA origami tiles followed by the washing step enabled multi-origami assemblies with higher yield and purity compared to any existing approaches. We envision this method to complement existing approaches to extend what is possible to construct with DNA.

Advisors: Grigory Tikhomirov

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BibTeX citation:

@mastersthesis{Kim:EECS-2023-237,
    Author= {Kim, Myoungseok and Tikhomirov, Grigory},
    Title= {Sequential Self-assembly of DNA-based Nanoscale Platform for the Fabrication of Nanophotonics},
    School= {EECS Department, University of California, Berkeley},
    Year= {2023},
    Month= {Nov},
    Url= {http://www2.eecs.berkeley.edu/Pubs/TechRpts/2023/EECS-2023-237.html},
    Number= {UCB/EECS-2023-237},
    Abstract= {Structural DNA nanotechnology has emerged as a versatile approach to building nanometer-precise structures for arranging non-DNA optical nanomaterials. To enable complex optical functions, systematic assembly rules for DNA assemblies into 2D and 3D nanostructures have been developed. However, the low purity and yield of the target superstructure is a major limitation for further development and practical applications. Here, inspired by oligonucleotide synthesis on the solid support, we propose a sequential assembly of DNA structures using the magnetic beads. The iterative addition of DNA origami tiles followed by the washing step enabled multi-origami assemblies with higher yield and purity compared to any existing approaches. We envision this method to complement existing approaches to extend what is possible to construct with DNA.},
}

EndNote citation:

%0 Thesis
%A Kim, Myoungseok 
%A Tikhomirov, Grigory 
%T Sequential Self-assembly of DNA-based Nanoscale Platform for the Fabrication of Nanophotonics
%I EECS Department, University of California, Berkeley
%D 2023
%8 November 21
%@ UCB/EECS-2023-237
%U http://www2.eecs.berkeley.edu/Pubs/TechRpts/2023/EECS-2023-237.html
%F Kim:EECS-2023-237